polymerase相关论文
24. The transcription factors and the relevant signaling pathways activated by low concentration MNN
Aims: To explore the transcription factors and related signal transduction pathways activated in the alkylating agents N......
乙型肝炎病毒(HBV)是导致肝炎、肝硬化等肝脏疾病的重要病原体。HBV前基因组RNA(pgRNA)在其生命周期中发挥着重要作用,它既是翻译病毒核......
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Although the detection of viral particles by reverse transcription polymerase chain reaction(RT-PCR)is the gold standard......
Although a live attenuated vaccine is available for controlling mumps virus(MuV), mumps still outbreaks frequently world......
A signal-on electroluminescence biosensor for mRNA based on polymerase assisted signal amplification
A novel electroluminescence biosensor was proposed in this paper.A novel strategy based on isothermal strand-displac......
目的探讨口服避孕药(Oral Contraceptive,OC)暴露、纤溶酶原激活物抑制剂1(Plasminogen Activator Inhibitor-1,PAI-1)基因多态性......
用聚合酶链,反应(PCR ) 基于方法六不同候选人疾病从米饭的抵抗基因(R) 相当或相同的事物被孤立。米饭序列与对比 genomic 组织模式......
NGF is one of the few endogenous neurotrophic factors which have been clarified in structure and gene sequence. The β s......
目的 探讨并建立粘多糖贮积症Ⅱ型(mucopolysaccharidosis Ⅱ,MPSⅡ)患者艾杜糖-2-硫酸酯酶(iduronate-2-sulphatase,IDS)基因常见突......
引起男性不育的原因很多,如自身抗体、解脲支原体等,而沙眼衣原体(Chlamydiatrachomatis CT)感染与男性不育的关系尚未完全明确,......
The levels of DNA polymerase α,δ,ε were examined in the neonatal mouse forebrains andspleens.The levels of DNA polyme......
YAC-base transfer of fibroin gene from Anthemea yamamal to domestic silkworm Bombyx mod (I)——Identif
Antheraea yamamai (Japanese oak silkworm) is a kind of silkworm of great economic value. and the process of the expressi......
T cell receptor TCR γ gene rearrangement in a series of acute lymphocytic leukemia (ALL) patients was studied using PCR......
聚合酶链反应(Polymerase Chain reac-tion,PCR)是由 Kary Mullis 等建立的一种体外核酸扩增方法,它是基于碱基互补的原则,以待扩......
PA_C subunit from avian influenza(H5N1) viral RNA polymerase was used in this work as a target in the screening for anti......
Transformation of Cabbage(Brassica oleracea L.var.capitata) with Bt cry1Ba3 Gene for Control of Diam
To obtain transgenic cabbage line with broad insect resistance,a new synthetic Bacillus thuringiensis cry1Ba3 gene was i......
Objective: To study the relationship of the mutation of HBV preS/S gene in HBsAg carrying pregnant women and intrauterin......
Study of Mutation in Tyrosine Protein Kinase of Insulin Receptor Gene in Patients with Polycystic Ov
Objective To explore the molecular mechanism of insulin resistance in the patients with polycystic ovarian syndrome (PC......
Polymerase chain reaction-single strand conformational polymorphism analysis of rearranged during tr
AIM: To investigate the relationship between mutations of rearranged during transfection (RET) proto-oncogene and Chines......
目的:了解S100B的mRNA及蛋白表达水平在良性前列腺增生症(BPH)合并2型糖尿病(T2DM)患者膀胱逼尿肌中的表达情况。方法:收集我院泌......
[目的]了解河南省艾滋病病毒-1型(human immunodeficiency virus-1,HIV-1)耐药毒株传播情况。[方法]通过横断面调查,采集年龄在16~6......
The present study enrolled 251 diabetic patients, including 101 with neuropathy and 150 without neuropathy. Of the 150 p......
Objective: To study the gene mutations of homeobox transcription factor ( CSX /NKX2.5) associated with a Chinese familyw......
BACKGROUND: The prevalences of hypertension, cerebrovascular diseases, etc. are higher in Mongolian population because o......
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Objective To evaluate the effects of polymorphisms in XRCC1 and APE1 genes on vinyl chloride(VC)-induced chromosomal dam......
AIM:To reverse multidrug resistance (MDR) of HepG2 byanti-MDR1 hammerhead ribozyme.METHODS:We developed an anti-MDR1 ha......
AIM:To synthesize three small-interference RNAs (siRNAs)by T7 RNA polymerase-catalyzed reaction,and to investigatetheir......
AIM:In recent years,studies have suggested that Epstein-Barr virus(EBV)is associated with HCC.The present studywas to d......
Identification of Rice Al-responsive Genes by Semi-quantitative Polymerase Chain Reaction using Sulf
Based on the evidence that Al resistance is an inducible process and rice is an Al-resistant crop,identification of Al-r......
Detection of minimal residual disease in leukemia using polymerase chain reaction and restriction an
DetectionofminimalresidualdiseaseinleukemiausingpolymerasechainreactionandrestrictionanalysisWuNanhai吴南海,LuShangen吕善根,ZhuPing...
DetectionofminimalresidualdiseaseinleukemiausingpolymerasechainreactionandrestrictionanalysisWuNanhai W......
目的 :本研究旨在分析牙龈卟啉菌基因型和其重要毒力因子胶原酶基因 (collagenasegene ,PrtC)的遗传异质性 ,了解细菌遗传变异与其......
[目的]通过改进的“自身模板引物”PCR法构建Grb-AST7基因串联体。[方法]根据蟋蟀神经肽Grb-AST7氨基酸序列设计合成2条引物,运用......
本文就乙型肝炎病毒、免疫、诊断、治疗及预防等方面的一些进展,作简要介绍。乙型肝炎病毒的核心一部分系由双股环形脱氧核糖核睃......
聚合酶链反应(Polymerase ChainReaction,简称PCR)是一种体外特异性扩增DNA的技术。它能将极微量的DNA样品特异地放大儿十万倍。......
病毒性肝炎应用聚合酶链反应(Polymerase Chain Reaction,PGR)研讨会,于1991年1月8~9日在广州第一军医大学举行。这项80年代中期建......
聚合酶链反应(Polymerase Chain Reaction),简称PCR,是新近建立的一种体外扩增特异性DNA的技术,具有快速、简便、敏感度高、特异......
Detection of Myobacterium Tuberculosis in the Samples of 122 TB Patients Using Polymerase Chain Reac
By means of polymerase chain reaction(PCR) technique,direct smear fluorescence microscopy and bacterial culture,the sput......
Objective To understand the deletion in the survival motor neuron gene (SMN) of childhood onset spinal muscular atro......
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应用聚合酶链式反应(Polymerase chain reaction,PCR)扩增了一例疫苗保护失败的乙型肝炎病毒持续携带者及其母亲产前血清中HBV S基因,......
聚合酶链反应(Polymerase ChainReaction,PCR)是一种在体外模拟自然DNA复制过程,用聚合酶催化物扩增核酸分子的新技术。由美国Cet......
ANALYSIS OF POINT MUTATION IN SITE 1896 OF HBV PRECORE AND ITS DETECTION IN THE TISSUES AND SERUM OF
Aim The 3’-base specific polymerase chain reaction (3’- BS- PCR) method was es-tablished to investigate the relations......
目的:利用副结核杆菌接种地鼠,以图建立一个模拟克隆病的小动物模型。方法:将40只地鼠随机分为两组,实验组:20只腹腔接种副结核杆......
Analysis of point mutation in site 1896 of HBV precore and its detection in the tissues and serum of
INTRODUCTION Hepatitis B is one of the common infectious diseases,which severely impairs the health of the people in o......
AIM To amplify HBV-RNase H gene fragment and expressionof RNase H for further use in the studies of HBV associatedliver ......